Isolasi Bakteri Asam Laktat (BAL) Penghasil Asam Glutamat dari Ikan Budu sebagai Feed Suplemen Ayam Broiler

V. Maslami, Y. Marlida, Mirnawati Mirnawati, Jamsari Jamsari, Y. S. Nur

Abstract


Asam glutamat merupakan asam amino building blocks asam amino lainnya dan neurotransmitter pembentuk citra rasa. Bakteri Asam Laktat (BAL) adalah salah satu mikrooganisme penghasil asam glutamat yang banyak terdapat pada produk fermentasi. Ikan budu merupakan fermentasi ikan Tenggiri (Scomberomorus guttatus) dan Talang-Talang (Chorinemus lyson L) yang diproduksi di daerah pesisir Kabupaten Padang Pariaman. Pada penelitian  ini dilakukan tiga tahap. Tahap 1: isolasi BAL yang berasal dari fermentasi ikan budu. Tahap 2: seleksi kualitatif dan kuantitatif BAL penghasil asam glutamat. Tahap 3: karakterisasi BAL penghasil asam glutamat. Hasil yang diperoleh terdapat 17 isolat BAL yang kemudian diseleksi secara kualitatif menggunakan thin layer chromatographic yang direndam dalam campuran pelarut n-butanol, asam asetat dan air (4:1:1 v/v) terdapat 16 yang dapat menghasilkan asam glutamat. Setelah dilakukan seleksi kualitatif selanjutnya dilakukan seleksi kuantitatif dengan spectrophotometer panjang gelombang 570 nm.  Produksi  asam glutamat yang tertinggi terdapat pada isolate IB.9  dengan produksi 13.03  mg/ml. Isolate  dan IB.9 yang paling tinggi dalam menghasilkan asam glutamat dikarakteristik yang berpedoman Bergey’s manual of systemic bacteriology  merupakan genus Lactobacillus sp. Penelitian ini dapat disimpulkan terdapat isolat BAL IB.9  (Lactobacillus sp) yang berpotensi dalam menghasilkan asam glutamat dengan produksi 13.03 mg/ml.


Keywords


asam glutamat; ayam broiler; BAL; ikan Budu; fermentasi

References


Ajinomoto Animal Nutrition. 2007. Influence of Glutamic Acid on Broiler Carcass Quality.

Aman, Y. 2011. Ayam Kampung Unggul. Penerbit Penebar Swadaya, Jakarta.

Berres J, S. L. Vieira , W. A. Dozier III , M. E. M. Cortês ,R. de Barros , E. T. Nogueira and M. Kutschenko. 2010. Broiler responses to reduced-protein diets supplemented with valine, isoleucine, glycine, and glutamic acid. Poultry Science Association, Inc. Vol. 19 :68–79.

Collins, C. H and D. M. Lyne. 1976. Microbiological methold. pp. 238-253.

Cullimore, R. D. 2000. Principal Atlas For Bacterial Identification. Lewis Publisher. United States of America.

Direktorat Jenderal Peternakan. 2015. Statistik Peternakan. Direktorat Jenderal Peternakan, Jakarta.

Fujimura, S., H. Koga., H. Takeda., N. Tone., M. Kadowaki, and T. Ishibashi. 1996. Role of taste-active components, glutamic acid, 5′-inosinic acid and potassium ion in taste of chicken meat extract. Animal Science and Technology 67, 423–429.

Fujimura, S., F. Sakai, and M. Kadowaki. 2001. Effect of restricted feeding before marketing on taste active components of broiler chickens. Journal of Animal Science 72: 223–229.

Gordon, R. E., Haynes, W. C, and Pang, C. H. 1973. Thr genus Bacillus. Wanghiton DC. US department of agriculture. Agricultural Handbook. Hal.42.

Imanari,M., Kadowaki and Fujimura. 2004. Regulation of taste-active components of meat bg diatary branchedchain amino acids. Proc.50th int/ cong.Meat Sci.Technol : 1276-1277.

Kasih, N. S. 2012. Pengaruh Lama Penyimpanan Daging Ayam Segar Dalam Refrigator Terhadap pH, Susut Masak, dan Organoleptik. Skripsi. Fakultas Pertanian. Universitas Islam Kalimantan Muhammad Aryad Al Banjary. Banjarmasin.

Kato, H and T. Nishimura. 1987. Taste components and condition of beef, pork and chicken. In: Kawamura Y, Kare RM (eds), Umami, A Basic Taste, pp. 289–306. Marcel Dekker, New York. Kong SE, Hall JC

Kiboem, L., J. Lee., Y. H. Kim., S. H. Moon, and Y. H. Park. 2001. Unique properties of four Lactobacillus in amino acid production and symbiotic mixed culture for lactic acid biosynthesis. Gun. Microb. Vol 43 : 123-131.

Kondoh, T., H. N. Mallick, and K. Torii. 2009. ctivation of the gut-brain axis by dietary glutamate and physiologic significance in energy homeostasis. Am. J. Clin. Nutr. Vol: 90, 832S–837S.

Lawal, A. K., Oso, B. A., Sanni, A. I, and Olatunji, O. O. 2011. L-Glutamic acid production by Bacillus spp. Isolated from vegetable proteins. African Journal of Biotechnology . 10: 5337-5345.

Lawrie, R. and D.H. Ledward. 2006. Lawrie’s Meat Science, 7th edn. Woodhead, Cambridge. assessment of the isolates for industrial potential. Bioresour. Technol. 2007, 98, 1380–1385.

Ogbadu, L. J., Okagbue, R. N, and Ahmed, A. A. 1990. Glutamic acid production by Bacillus isolates from fermented vegetable proteins. J. Microbiol. 6: 377-382.

Pierre-Andre, G and M. Yves. 2004. Amino Acids: Beyond the Building Blocks Poult. Sci 83:650-657.

Purwohadisantoso, K., Zubaidah, E, and Saparianti, T. 2009. Isolation of lactic acid bacteria from cabbage and their potensial inhibition to pathogenic bacteria (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella thypimurium). Jurnal Teknologi Pertanian 10(1): 19-27.

Ray, B. 2001. Dasar–dasar Mikrobiologi Pangan. Diterjemahkan oleh Rindit Pambayun dan Rahmat Hari Purnomo. Jurusan Teknologi Pertanian. Fakultas Pertanian Universitas Sriwijaya.

Sano, C. 2009. History of glutamate production. Am. J. Clin. Nutr. 90, 728S–732S.

Sneath, P. H. A., Mair., Sharpe M. E., Holt, J.G. 1986. Bergey’s Manual of Systematic Bacteriology; Williams and Wilkins ; Baltimore. MD. USA.

Spies, J. R. 1957. Colorimetric procedures for amino acids methods in Enzymology. 3: 468-471

Tarek., M. El-Nemr, and H. E. Mostafa. 2010. Screening Of potensial infans Ilactobacili isolat for amino acids production.Alexandria. African Jurnal Of Microbiology research vol. 4 (4),pp. 226-232.

Winarno, F. G. 1990. Teknologi Fermentasi. Proyek Pengembangan Pusat Fasilitas bersama . Antar Universitas, PAU Pangan dan Gizi, UGM, Yogyakarta.

Winarno, F. G. 2004.Kimia Pangan dan Gizi. Gramedia Pustaka Utama. Jakarta.

Young, J. F., A. H. Karlsson, and P. Henckel. 2004. Waterholding capacity in chicken breast muscle is enhanced by pyruvate and reduced by creatine supplements. Poultry Science 83: 400–405.

Young, V.R and A.M. Ajami AM. 2000. Glutamate: an amino acid of particular distinction. Journal of Nutrition. Vol .130: 892S–900S.

Yusra., A. Fauzan., Novelina, dan Periadnadi. 2014. Isolasi dan identifikasi mikroflora indigenous dalam budu. Agritech : 34

Zalan, Z., Hudacek, J., Stetina, J., and Chumchalova, J. 2010, Production of organic acids by Lactobacillus strains in three different media. Eur. Food Res. Technol. 230, 395–404.

Zhang, G. Q., Q. G Ma and Ji C. 2008. Effects of dietary inosinic acid on carcass characteristics, meat quality, and deposition of inosinic acid in broilers. Poult. Sci

Zareian, M., Ebrahimpour, A., Bakar, F.A., Mohamed, A. K. S., B. Forghani., M. S. B. Ab-Kadir, and Saari, N. 2012. A glutamic acid-producing lactic acid bacteria isolated from Malaysian Fermented foods. International Journal of Molecular Sciences.




DOI: https://doi.org/10.25077/jpi.20.1.29-36.2018

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