Pengaruh NaCl Fisiologis dan Ringer Laktat terhadap Kualitas Spermatozoa pada Suhu Ruang dan Fertilitas Telur Ayam Buras
Abstract
Kualitas spermatozoa menentukan keberhasilan Inseminasi Buatan (IB). Pengencer sederhana yang memiliki sifat buffer dan isotonik dapat digunakan sebagai pengencer semen ayam. Tujuan penelitian ini adalah membandingkan kualitas spermatozoa ayam buras dalam pengencer Natrium chlorida (NaCl) Fisiologis dan Ringer Laktat (RL) pada penyimpanan suhu ruang dan menguji fertilitas telur berdasarkan lama simpan terbaik. Penelitian ini menggunakan semen yang berasal dari 2 pejantan ayam buras, penyimpanan semen selama 2 jam pada suhu ruang dengan interval pengamatan 30 menit. Inseminasi dilakukan pada 20 ekor betina ayam buras. Parameter yang diamati adalah motilitas, viabilitas dan abnormalitas spermatozoa. Analisis statistik kualitas semen dengan Analysis of Variant (ANOVA) pola tersarang dan analisis fertilitas telur dengan uji chi-square. Hasil penelitian menunjukkan bahwa penambahan pengencer NaCl Fisiologis dan RL berpengaruh nyata (P<0,05) terhadap penurunan kualitas semen selama penyimpanan suhu ruang. Lama simpan terbaik pada suhu ruang yang masih mampu mempertahankan kualitas semen adalah pada menit ke-30 dan hasil ini digunakan untuk aplikasi IB dengan fertilitas telur berbeda nyata (P<0,05) masing-masing perlakuan yaitu 82,61% (NaCl Fisiologis) dan 60,01% (RL). Kesimpulan dari penelitian ini bahwa kualitas spermatozoa ayam sampai menit ke-30 pada suhu ruang masih memenuhi syarat untuk aplikasi IB, dan fertilitas telur terbaik pada penggunaan pengencer NaCl Fisiologis.
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ddo, A. J. A. Hamidu., A. Y. Ansah, and K. Adomako. 2018. Impact of Egg Storage Duration and Temperature on Egg Quality, Fertility, Hatchability and Chick Quality in Naked Neck Chickens. International Journal of Poultry Science.17: 175-183.
Ax, R. L., M. R. Dally., B. A. Didion., R. W. Lenz., C. C. Love., D. D. Varner., B. Hafez, and M. E. Bellin. 2008. Semen Evaluation in Farm Animal Reproduction ed by Hafez ESE. 7 th edition. Lea and Febiger: 365-375.
Bakst, M. R. and J. S. Dyamond. 2013. Artificial Insemination in Poultry in Succes in Artificial Insemination. Edited by Alemayehu Lemma. INTECH: 176-195.
Boleli, I. C., V. S. Morita., Jr. J. B Matos., M. Thimotheo, and V. R. Almeida. 2016. Poultry Egg Incubation: Integrating and Optimizing Production Efficiency. Brazilian Journal of Poultry Science.2: 1-16.
Brillard, J. P. 2003. Practical aspects of fertility in poultry. World Poult. Sci. J. 59: 441-446.
Chelmonska, B., A. Jerysz., E. L. Ukaszewicz., A. Kowalczyk, and I. Malecki. 2008. Semen Collection from Japanese Quail (Coturnix japonica) Using a Teaser Female.Turk.J. Vet. Anim. Sci.32(1): 19-24.
Danang, D. R., N. Isnaini, and P. Trisunuwati. 2012. Save Old influence on the Quality of Sperm Semen Native Chicken In Ringer’s diluent at 4 ° C. Jurnal Ternak Tropika . 13(1): 47-57.
Donoghue. A. M. and G. J. Wishart. 2000. Storage of poultry semen. Animal Reproductive Science.62: 213-232.
Fikremariam, F. and A. Tilahun. 2016. Fertility and Hatchability of Fayoumi and Dominant Breeds of Chicken in Debere Zeit Agricultural Research Centre, Ethiopia. Academia Journal of Agricultural Research, 4(5): 241-246.
Garner, D. L. and E. S. E. Hafez. 2008. Spermatozoa and Seminal Plasma in Reproduction In Farm Animals. Edited by E. S. E. Hafez and B. Hafez . 7th edition. Lea and Febiger. Philadelphia: 167-188.
Getachew, T. 2016. A Review Article of Artificial Insemination in Poultry. World’s Veterinary Journal. 6 (1): 26-35.
Habibullah, M., M. A. Hashem, M. S. Rana, and M. H. Islam. 2015. Effect of Artificial Insemination on different production parameter in Hubbard classic broiler parent stock. J. Bangladesh Agril. Univ. 13(1): 71–77.
Hafez, E. S. E. 2008. Artificial insemination. In E.S.E. Hafez. Ed. Reproduction in Farm Animals. 7th edition. Lea and Febiger. Philadelpia.
Hudson, G. H., A. V. Omprakash, and K. Premavalli. 2016. Effect of Semen Diluents, Dilution Rates and Storage Periods on Live and Abnormal Spermatozoa of Pearl Guinea Fowls. Asian Journal of Animal and Veterinary Advances. 11: 411-416.
Iswati., N. Isnaini, dan T. Susilawati. 2017. Fertilitas Spermatozoa Ayam Buras dengan Penambahan Antioksidan Glutathione dalam Pengencer Ringer’s Selama Simpan Dingin. Jurnal Ilmu-Ilmu Peternakan 27 (1): 107 – 115.
Iswati., N. Isnaini, and T. Susilawati. 2018. Effect of Addition of Glutathione In Diluent Ringer’s on Spermatozoa Quality of Domestic Chicken During Cold Storage. Asian Journal of Microbiology Biotechnology Environment. Sceince. 20(1): 12-20.
Lukaszewicz, E. 2001. Effects of semen filtation and dilution rate on morphology and fertility of frozen gander spermatozoa. Theiogenology. 55: 1819-1829.
Lukaszewicz, E. 2002. An effective method for freezing White Italian gander semen. Theriogenology. 58:19-27.
Madeddu, M. L. Zaniboni., M. G. Mangiagalli, C. Cassinelli, and S. Cerolini. 2013. Egg related parameters affecting fertility and hatchability in the Italian bantam breed Mericanel della Brianza. Animal Reproduction Science 137: 214–219.
Modupe, O., A. C Livinus, and N. B. Ifeany. 2013. Semen Quality Characteristic and Effect of Mating Ratio on Reproductive Performance of Hubbarrd Broiller Bredders. Journal of Agriculture Science. 5(1) 154-159.
Mohan, J., Sharma, S. K., KollurI, G., Singh, R. P., Tyagi, J. S. and Kataria, J. M. 2015. Selection od Diluent for short Term Preservation of Guinea Fowl Semen. Asian Journal of Animal and Veterinary Advances. 10(8): 360-364.
Mohan, J., Sharma, S. K., KollurI, G., Singh, R.P., Tyagi, J.S. and Kataria, J.M. 2016. Semen quality characteristics and seasonality in different varieties of male guinea fowl. Advances in Animal and Veterinary Sciences.4 (6): 320-326.
Mohan, J., Sastry, K.V.H. and Kataria, J.M. 2017. A Process for the Preparation of CARI Poultry Semen Diluent’ in patent office New Delhi. Patent filed on 28/02/2017, Application number 201711007119.
Mohan, J., K. Sharma, G. Kolluri and K. Dhama. 2018. History of Artificial Insemination in Poultry, It’s Components and Significance. World’s Poultry Science Journal. 74: 1-14.
Nahak, A.K., Giri, S.C., Mohanty, D.N., Mishra, P.C. and Dash, S.K. 2015. Effect of frequency of collection on seminal characteristics of White Pekin duck. Asian Pacific Journal of Reproduction. 4 (1): 70-73.
Peters SO, O.D. Shoyebo, B.M. Ilori, M.O. Ozoje, C.O.N. Ikeobi and O.A. Adebambo. 2008. Semen quality traits of seven strain of chickens raised in humid tropics. International Journal of Poultry Science. 7: 949-953
Ridwan dan Rusdin. 2008. Konservasi Semen Ayam Buras Menggunakan Berbagai Pengencer Terhadap Fertilitas Dan Periode Fertil Spermatozoa Pasca Inseminasi Buatan.J. Agroland.15 (1) : 63 – 67.
Siudzin´ska, A and E. Łukaszewicz. 2008. Effect of Semen Extenders and Storage Time on Sperm Morphology of Four Chicken Breeds. J. Appl. Poult. Res. 17:101–108
Susilawati, T. 2011. Spermatology. UB Press. Malang.
Tabatabaei, S. 2010. The effect Spermatozoa Number on Fertility Rate of Chicken in Artificial Insemination Programs. Journal of Animal and Veterinary Advances. 9(12): 17-19.
Tarif, A.M.M., M. M.U. Bhuiyan, R. N. Ferdousy, N. S. Juyena and M. B. R. Mollah. 2013. Evaluation of semen quality among four chicken lines. IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) 6 (5): 7-13.
Tangpakdeewijit, S., S. Ponchunchoovong and T. Vongpralub. 2015. Effect of extenders on frozen semen quality of Thai native chicken (Lueng hang kao). Khon Kaen Agr. J. 43 Suppl. 2 .
Vasicek, J., L.Kuzelova, B. Kulikova, and P. Chrenek. 2015. Effect of diluent and storage time on sperm characteristics of rooster insemination doses. Avian Biology Research 8 (1): 41-26.
Yamte, K., B. Wantow, E.de Queljoe. 2013. Perbedaan motilitas Spermatozoa Sapi Jantan (Frisian holstein) setelah pemberian cairan Kristaloid Ringer Laktat. Jurnal e-Biomedik 1(1): 185-189.
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