Keragaman Baru pada Daerah Ujung Gen Hormon Pertumbuhan Sapi Pesisir Ternak Lokal Sumatera Barat

Yurnalis Yurnalis, Arnim Arnim, Sarbaini Sarbaini, Jamsari Jamsari

Abstract

Dari 210 sampel sapi pesisisir umur 1,5 tahun, dipilih 60 sample berdasarkan berat badan yaitu 30 sampel dengan berat tinggi (125±9,02 kg) dan 30 sampel dengan berat badan rendah (65±6,09 kg). Sampel darah 60 ekor sapi diisolasi dan produk PCR dari fragmen gen GH (455 bp)  disekuensing forward dan reserve.  Dua dilesi ditemukan pada posisi 2731 dan 2732 dengan fekuensi allel 0.06, dan 0.19. Delapan mutasi ditemukan pada posisi  2537, 2567, 2639, 2647, 2706, 2713, 2732, dan 2813 dengan genotipe  G → T,  G → T, C → G, T → C, A → G, C → T, C → T, C → A dengan frekuensi allel berturut-turut 0,66; 0,97; 1,00; 0,54; 0,37; 0,62; 0,77 dan 1,00. Hasil penelitian ini menunjukkan gen GH pada sapi pesisir mempunyai keragaman yang tinggi, sehingga perlu dilakukan penelitian lanjut untuk mengetahui apakah keragaman ini berhubungan dengaan sifat-sifat produksi.

 Keywords

Sapi Pesisir; gen GH; Keragaman; SNP

 Full Text:

PDF (Bahasa Indonesia)

References

Falaki, M., N. Gengler, M. Sneyers, A. Prandi, S. Massart, A. Formigoni, A. Burny, D. Portetelle and R. Renaville. 1996. Relationships of polymorphisms for growth hormone and growth hormone receptor genes with milk production traits for Italian Holstein-Friesian bulls. J. Dairy Sci. 79:1446–1453.

Beauchemin, V. R., M. G. Thomas, D. E. Franke and G. A. Silver. 2006. Evaluation of DNA polymorphisms involving growth hormone relative to growth and carcass characteristics in Brahman steers. Genet. Mol. Res 5:438-447.

Ge, W., M. E. Davis, H. C. Hines, K. M. Irvin and R. C. M. Simmen. 2003. Association of single nucleotide polymorphisms in the growth hormon and growth hormon receptor genes with blood serum insulin-like growth factor I concentration and growth traits in Angus cattle. J. Anim. Sci. 81:641–648.

Gordon, D. F., D. P. Quick, C. R. Erwin, J. E. Donelson and R. A. Maurer. 1983. Nucleotide sequence of the bovine growth hormone chromosomal gene. Mol. Cell. Endocrinol. 33:81–95.

Hediger, R., S. E. Johnson, W. Barendse, R. D. Drinkwater, S. S. Moore and J. Hetzel. 1990. Assignment of the growth hormone gene locus to 19q26-qter in cattle and to 11q25-qter in sheep by in-situ hybridization. Genomics 8:171–174.

Hale, C. S., W. O. Herring, H. Shibuya, M. C. Lucy, D. B. Lubahn, D. H. Keisler and G. S. Johnson. 2000. Decreased growth in Angus steers with a short TG-microsatellite allele in the P1 promoter of growth hormone receptor gene. J. Anim. Sci. 78:2099– 2104.

Lagziel, A., E. Lipkin, E. Ezra, M. Soller and J. I. Weller. 1999. An MspI Polymorphism at the bovine growth hormone (bGH) gene is linked to a locus affecting milk protein percentage. Anim. Genet. 30:296-299

Matsuhashi, T., S. Maruyama, Y. Uemoto, N. Kobayashi, H. Mannen, T. Abe, S. Sakaguchi and E. Kobayashi. 2011. Effects of bovine fatty acid synthase, stearoyl-coenzyme A desaturase, sterol regulatory element-binding protein 1, and growth hormone gene polymorphisms on fatty acid composition and carcass traits in Japanese Black cattle. J. Anim. Sci. 2011. 89:12–22

Maylinda, S. 2011. Genetic polymorphism of growth hormone locus and its association with bodyweight in Grati dairy cows. International Journal for Biotechnology and Molecular Biology Research Vol. 2(7):117-120.

Moravčíková, N., A. Trakovická and E. Hazuchová. 2012. The Association of Bovine Growth Hormone Gene Polymorphism with Milk Performance Traits in Slovak Spotted Cows. Scientific Papers: Animal Science and Biotechnologies. 45:20-210.

Pierzchala, M., T. Blicharski and J. Kuryl. 2004. Growth rate and carcass quality in relation to GHlMspl and GHlHaell PCR-RFLP polymorphism in pig Animal Science Papers and Report 22:57-64.

Putra, D. E., Sumadi, T. Kanazawa and T. Hartatik. 2015. Short Communication: Identification of Growth Hormone gene polymorphism for beef cattle in Pesisir Selatan District, West Sumatra, Indonesia. Biodiversitas. 17(2): 711-715.

Reis, C., D. Navas, N. Pereira and A. Cravador. 2001. Growth hormon Alul polymophism analysis in eight Portuguese bovine breeds Arch. Zootec. 50:41-48.

Sami, A. J. 2009. Deletion of amino acid residues 33-46 in growth hormone alters the hydrophobicity of the molecule. African Journal of Biotechnology 9 (5): 711-717

Sami, A. J., M. T. Nazir, Z. Jabeen and A. R. Shakoori. 2011. Gene study within the 5' flanking regions of growth hormone gene of first exon in Bos indicus. African Journal of Biotechnology 10(3): 332-336.

Tambasco, D. D., C. C. P. Paz, M. T. Stuart, A. P. Pereira, M. M. Alencar, A. R. Freitas, L. L. Coutinho, I. U. Packer and L. C. A. Regitano. 2003 Candidate genes for growth traits in beef cattle crosses Bos taurus x Bos indicus. Abstract. J. An. Breeding and Genetics. 120:51

Tatsuda, K., A. Oka, E. Iwamoto, Y. Kuroda, H. Takeshita, H. Kataoka, and S. Kouno. 2008. Relationship of the Bovine Growth Hormone Gen to Carcass Traits in Japanese Black Cattle. J. Anim. Breed. Genet. 125 : 45 - 49

Woychik, R. P., S. A. Camper, R. H. Lyons, S. Horowitz, E. C. Goodwin and F. M. Rottman. 1982. Cloning and nucleotide sequencing of the bovine growth hormone gene. Nucleic Acids Res. 10:7197–7210.

Yao, J. B., S. E. Aggrey, D. Zadworny, J. F. Hayes and U. Kuhnlein. 1996. Sequence variations in the bovine growth hormone gene characterized by single-strand conformation polymorphism (SSCP) analysis and their association with milk production traits in Holsteins. Genetics 144:1809–1816.

Yardibi, H., H. T. Hosturk, I. Paya, F. Kaygisiz, G. Ciftioglu, A. Mengi and K. Oztabak. 2009. Associations of Growth Hormone Gene Polymorphisms with Milk Production Traits in South Anatolian and East Anatolian Red Cattle. J. Anim. Vet. Adv., 5: 1040-1044.

Yurnalis, Sarbaini, Arnim, Jamsari, Wolgang Nellen. 2013. Identification Single Nuleotide Polymorphism of Growth Hormone Gene Exon 4, Intron 4 in Sapi pesisir Local Cattle breeds in West Sumatera Province of Indonesia. African Journal of Biotechnology. Vol 12(3).

Zakizadeh, S., G. Rahimi, S. R. Mirae-Ashtiani, A. Nejati-Javaremi, M. Moradi-shahrbabak, P. Reinecke, M. Reissmann, A. A. Masoudi, C. Amirinia and S. A. Mirhadi. 2006. Analysis of Bovine Growth Hormone Gene Polymorphism in Three Iranian Native Breeds and Holstein Cattle by RFLP-PCR. Biotechnology 5(3): 385-390.

Zhang, H. M., D. R. Brown, S. K. Denise, and R. L. Ax. 1993. Rapid communication: polymerase chain reaction-restriction fragment lenght polymorphism analysis of the bovine somatotropin gen. J. Anim. Gent. 71:2276.

Zhou, G. L., H. G. Jin, C. Liu, S. L. Guo, Q. Zhu and W. Y. Hou. 2005. Associated of genetic polymorphism in GH gene with milk production traits in Beijing Holstein cows. J. Biosci. 30: 595-598.

DOI: https://doi.org/10.25077/jpi.19.3.103-109.2017

Refbacks

  • There are currently no refbacks.