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Home > Vol 13, No 1 (2011) > Syaiful
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F. L. Syaiful
Indonesia

R. Saladin
Indonesia

Jaswandi Jaswandi
Indonesia

Z. Udin
Indonesia

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Keywords Bacillus amyloliquefaciens Halaman Belakang Halaman Depan ayam broiler broiler daging fermentasi in vitro karkas kunyit pH pendapatan performa potensi probiotik produktivitas protein protein kasar sapi perah sapi potong sinbiotik
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Pengaruh Waktu Fertilisasi dan Sistem Inkubasi yang Berbeda terhadap Tingkat Fertilisasi Sapi Lokal Secara In Vitro

F. L. Syaiful, R. Saladin, Jaswandi Jaswandi, Z. Udin

Abstract

The purpose of this study was to determine the effect of fertilization time and different incubation systems on fertilization level by in-vitro. The mature of ovaries from indigenous cow and fresh cement from Holstein Frisian cows (FH), 0.9 % NaCl physiological PBS, Nissui Japan, l,TCM-199, HEPES 30 μM, 20 mL Heparin, 10% goat serum, 250 g/ml FSH BO medium, mediumgentamisain 50 mB-O, mineral oil, alcohol, aquabidest, and 1% aceto orcein were materials and reagents. The Completely Randomized Design (CRD) in factorial pattern was used. Results shown no significant effect on the percentage of fertilized oocytes and level of development of pronuclei (2PN and> 2PN) by different timing of fertilization and incubation system. The development of pronucleus (1PN) showed significant (P <0.05) on 12 hours (37.60 %), but no significant effect on different incubation system. It concluded, the system of incubation and time of fertilization has no effect on oocyte fertilization rate. Oocytes fertilization time can be performed at 6 hours, 12 hours, and 18 hours, while the extension of the period of fertilization until 18 hours did not increase the level of fertilization.

 Keywords

Incubation systems; fertilization time; FIV and pronucleus

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DOI: https://doi.org/10.25077/jpi.13.1.27-35.2011

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Published by Faculty of Animal Science Universitas Andalas associated with Indonesian Association of Nutritionist and Feed Scientist

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ISSN (Online) 2460-6626

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JURNAL PETERNAKAN INDONESIA

JURNAL PETERNAKAN INDONESIA

JURNAL PETERNAKAN INDONESIA

JURNAL PETERNAKAN INDONESIA

JURNAL PETERNAKAN INDONESIA

JURNAL PETERNAKAN INDONESIA

JURNAL PETERNAKAN INDONESIA

JURNAL PETERNAKAN INDONESIA


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Jurnal Peternakan Indonesia (JPI) 

Fakultas Peternakan Universitas Andalas.
Kampus Unand Limau Manis Padang-25163, Sumatera Barat, Indonesia.