The objectives of this experiment were to study the effect of chicken hypophyse gland exttact for spawning of carp. Each twenty female of about 2950-3050 g/head and 100 of male carp of about 300-350 g body weight/head were used in the experiments. Hypophyse gland extract were prepared from the hypophyse glands of broiler chickens of about 10 days old. As treatments, the extract was injected in five different doses of 200, 400, 600, 800 and 1000 mg/kg body weight. Each dose was injected twice. The second injection was done after 1 hours. Each treatment consisted of 4 replications as block. Parameter measured included: egg fertility, egg hatching rate and survival rate of larvae, water qualities (temperature, dissolved 02. free CO2, NH3 content and pH). Data were statistically analyzed by variance analysis in randomized block design (RBD). Results indicated that injection of broiler's hypophyse gland extract gave highly significant (P < 0.01) effect on spawning egg fertility, egg hatching rate and survival rate of carp larvae of three days old. The optimal injection dose was found 582 mg broiler's hypophyse gland/kg carp body weight.